Journal: Journal of Lipid Research

Article Title: High binding affinity of electronegative LDL to human aortic proteoglycans depends on its aggregation level

doi: 10.1194/jlr.m800318-jlr200

Figure Lengend Snippet: Fig. 4. Apolipoprotein E (apoE) and apoC-III content in LDL(1), total LDL(2), and LDL(2) subfractions. LDL(2) was subfrac- tionated depending on its PG affinity (see Fig. 3A). ApoE and apoC-III were determined as described in Materials and Methods. Results are the mean 6 SD of five independent experiments. * P , 0.05 versus LDL(1); # P , 0.05 versus LDL(2) 1 subfraction; † P , 0.05 versus LDL(2) 2 subfraction; ‡ P , 0.05 versus LDL(2) 3 subfraction.

Article Snippet: Polyclonal rabbit anti-human apoE IgG (R107, IgG purified by Protein G-method) was coupled to CNBr-activated Sepharose CL4B according to the manufacturerʼs instructions (Amersham Pharmacia Biotech).

Techniques:

Journal: Journal of Lipid Research

Article Title: High binding affinity of electronegative LDL to human aortic proteoglycans depends on its aggregation level

doi: 10.1194/jlr.m800318-jlr200

Figure Lengend Snippet: Fig. 5. Analytical PG affinity chromatography of apoE-rich LDL (2) and apoE-poor LDL(2). ApoE-rich and apoE-poor LDL(2) subfractions were obtained by anti-apoE immunoaffinity chroma- tography, as described in Materials and Methods. Samples were concentrated and injected in the PG analytical affinity chroma- tography column. Figure shows a representative experiment. Dashed line indicates NaCl gradient.

Article Snippet: Polyclonal rabbit anti-human apoE IgG (R107, IgG purified by Protein G-method) was coupled to CNBr-activated Sepharose CL4B according to the manufacturerʼs instructions (Amersham Pharmacia Biotech).

Techniques: Affinity Chromatography, Injection